Protein engineering /

Other Authors: Robertson, Dan E., Noel, Joseph P.
Format: Book
Language: English
Published: Amsterdam ; Boston : Elsevier Academic Press, 2004
Series: Methods in enzymology ; v. 388
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Table of Contents:
  • Section I. Methodology
  • Gene site saturation mutagenesis : a comprehensive mutagenesis approach
  • Single-stranded DNA family shuffling
  • Module shuffling
  • SCHEMA-guided protein recombination
  • Staggered extension process in vitro DNA recombination
  • Using incremental truncation to create libraries of hybrid enzymes
  • Random multirecombinant polymerase chain reaction
  • Gene library synthesis by structure-based combinatorial protein engineering
  • New enzymes from combinatorial library modules
  • Construction of protein fragment complementation libraries using incremental truncation
  • Section II. Applications: optimization and screening
  • GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform
  • High throughput microplate screens for directed protein evolution
  • Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities
  • Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis
  • Screening for oxidative resistance
  • Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes
  • Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system
  • Section III. Applications: directed evolution of enzymatic function
  • Directed evolution of lipases and esterases
  • Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium
  • Directed evolution of aldolases
  • Changing the enantioselectivity of enzymes by directed evolution
  • Control of stereoselectivity in phosphotriesterase
  • Section IV. Applications: evolution of biosynthetic pathways
  • Manipulation and analysis of polyketide synthases
  • Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases
  • Engineering carotenoid biosynthetic pathways
  • Section V. Devices, antibodies, and vaccines
  • Directed evolution of bacteriorhodopsin for device applications
  • Engineering antibody affinity by yeast surface display
  • A conditionally replicating virus as a novel approach toward an HIV vaccine.