Protein engineering /

Other Authors: Robertson, Dan E., Noel, Joseph P.
Format: Book
Language: English
Published: Amsterdam ; Boston : Elsevier Academic Press, 2004
Series: Methods in enzymology ; v. 388
Subjects:
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245 0 0 |a Protein engineering /  |c edited by Dan E. Robertson, Joseph P. Noel. 
260 |a Amsterdam ;  |a Boston :  |b Elsevier Academic Press,  |c c2004. 
300 |a xxxvii, 413 p., [16] p. of plates :  |b ill. (some col.) ;  |c 24 cm. 
440 0 |a Methods in enzymology ;  |v v. 388 
504 |a Includes bibliographical references and indexes. 
505 0 |a Section I. Methodology -- Gene site saturation mutagenesis : a comprehensive mutagenesis approach -- Single-stranded DNA family shuffling -- Module shuffling -- SCHEMA-guided protein recombination -- Staggered extension process in vitro DNA recombination -- Using incremental truncation to create libraries of hybrid enzymes -- Random multirecombinant polymerase chain reaction -- Gene library synthesis by structure-based combinatorial protein engineering -- New enzymes from combinatorial library modules -- Construction of protein fragment complementation libraries using incremental truncation -- Section II. Applications: optimization and screening -- GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform -- High throughput microplate screens for directed protein evolution -- Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities -- Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis -- Screening for oxidative resistance -- Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes -- Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system -- Section III. Applications: directed evolution of enzymatic function -- Directed evolution of lipases and esterases -- Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium -- Directed evolution of aldolases -- Changing the enantioselectivity of enzymes by directed evolution -- Control of stereoselectivity in phosphotriesterase -- Section IV. Applications: evolution of biosynthetic pathways -- Manipulation and analysis of polyketide synthases -- Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases -- Engineering carotenoid biosynthetic pathways -- Section V. Devices, antibodies, and vaccines -- Directed evolution of bacteriorhodopsin for device applications -- Engineering antibody affinity by yeast surface display -- A conditionally replicating virus as a novel approach toward an HIV vaccine. 
650 0 |a Protein engineering. 
650 0 |a Proteins  |x Synthesis. 
650 0 |a Genetic engineering. 
650 0 |a Proteins  |x Structure-activity relationships. 
650 2 |a Genetic Engineering. 
650 2 |a Protein Conformation. 
650 2 |a Proteins  |x genetics. 
700 1 |a Robertson, Dan E. 
700 1 |a Noel, Joseph P. 
866 0 |8 0  |a v.388 
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