Chromatin and chromatin remodeling enzymes. Part B /

Other Authors: Allis, C. David, Wu, Carl.
Format: Book
Language: English
Published: San Diego : Elsevier/ Academic Press, 2004
Series: Methods in Enzymology, v. 376
Subjects:
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245 0 0 |a Chromatin and chromatin remodeling enzymes.  |n Part B /  |c edited by C. David Allis, Carl Wu. 
260 |a San Diego :  |b Elsevier/ Academic Press,  |c c2004. 
300 |a xxxviii, 458 p., [7] p. of plates :  |b ill. (some col.) ;  |c 23 cm. 
440 0 |a Methods in Enzymology,  |x 0076-6879 ;  |v v. 376 
504 |a Includes bibliographic references and index. 
505 2 |a 1. Fluorescence anisotrophy assays for analysis of ISWI-DNA and ISWI-nucleosome interactions -- 2. Biophysical analysis of specific genomic loci assembled as chromatin in vivo -- 3. Visualization and 3D structure determination of defined sequence chromatin and chromatin remodeling complexes -- 4. Electron microscopic analysis of the RSC chromatin remodeling complex -- 5. Use of optical trapping techniques to study single-nucleosome dynamics -- 6. Single-molecule analysis of chromatin -- 7. Biochemical and structural characterization of recombinant histone acetyltransferase proteins -- 8. Use of nuclear magnetic resonance spectroscopy to study structure-function of bromodomains -- 9. Assays for the determination of structure and dynamics of the interaction of the chromodomain with histone peptides -- 10. Expression, purification, and biophysical studies of chromodomain proteins -- 11. Quantitative assays for characterization of the Sir2 family of NAD+-dependent deacetylases -- 12. Selective HAT inhibitors as mechanistic tools for protein acetylation -- 13. Histone deacetylase inhibitors: assays to assess effectiveness in vitro and in vivo -- 14. Immunochemical analysis of chromatin -- 15. Generation and characterization of antibodies directed against di-modified histones, and comments on antibody and epitope recognition -- 16. Generation and characterization of methyl-lysine histone antibodies -- 17. Tips in analyzing antibodies directed against specific histone tail modifications -- 18. Histone methylation: recognizing the methyl mark -- 19. Analysis of genome-wide histone acetylation state and enzyme binding using dna microarrays -- 20. Use of chromatin immunoprecipitation assays in genome-wide location analysis of mammalian transcription factors -- 21. High-throughput screening of chromatin immunoprecipitates using CpG-island microarrays -- 22. Chromatin immunoprecipitate in the analysis of large chromatin domains across murine antigen receptor loci -- 23. The use of chromatin immunoprecipitation assays in genome-wide analyses of histone modifications -- 24. Sequential chromatin immunoprecipitation from animal tissues -- 25. Immunofluorescent staining of polytene chromosomes: exploiting genetic tools -- 26. Indirect immunofluorescent labeling of Drosophila polytene chromosomes: visualizing protein interactions with chromatin in vivo -- 27. X-chromosome inactivation in mouse embryonic stem cells: analysis of histone modifications and transcriptional activity using immunofluorescence and FISH -- 28. X inactivation in mouse ES cells: histone modifications and FISH. 
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700 1 |a Allis, C. David 
700 1 |a Wu, Carl. 
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